Researching ways to prevent more Listeria deaths

As the death count in South Africa’s listeriosis outbreak rises, Canadian researchers are isolating bacteria from the microbiome of exotic foods to try to develop a solution.

The largest Listeria food-borne outbreak ever recorded in human history is now taking place in South Africa.

According to the latest figures, 852 cases have been confirmed — 42 per cent of them in babies less than one month old — and 107 people have died.

Listeria is a scary bacterium because it can be found in your fridge. The most famous listeriosis outbreak in Canada occurred in 2008, when contaminated deli meat caused 57 illnesses and led to the deaths of 24 people.

The full name of the bacterium is Listeria monocytogenes and it causes foodborne illness and death mainly in at-risk individuals. These usually include pregnant women, individuals over 65 years of age, people like cancer patients who take medication to suppress their immune systems and those who are HIV-positive.

As director of the Canadian Research Institute for Food Safety, I am exploring the potential of microbiome research to combat Listeria outbreaks like the one occurring in South Africa right now.

There are also lessons that both individual consumers and the food industry in Canada can learn from previous outbreaks to prevent them from occurring in the future.

A widespread and hardy bacterium

There are a number of things that make Listeria monocytogenes unique and differentiate it from other foodborne pathogens.

First, this bacterium can actually grow at refrigeration temperatures, so refrigerating foods cannot be used as a strategy to combat this organism.

Second, it’s a very hardy organism and can resist chemicals such as salt much better than other food-borne pathogenic bacteria.

As a result of its hardiness, it can survive for months and even years in an environment such as a meat-processing plant. It can then go on to contaminate meat as it’s moving through the various steps in the manufacturing facility.

Third, it’s very widespread in the environment and can be found in many different places.

Finally, it can be a very deadly bacterium.

In fact, among all the food-borne pathogens, Listeria monocytogenes has the highest number of deaths that occur during a particular period of time, such as in an outbreak. The deli-meat outbreak in Canada had a fatality rate among confirmed cases of 42 per cent.

Food and other sources of Listeria

Foods commonly identified as sources of Listeria infection include cheeses (particularly soft-ripened varieties, such as traditional Mexican cheeses, Brie/Camembert and ricotta), ice cream, raw fruits and vegetables such as cantaloupe, packaged lettuce, bean sprouts, peaches and caramel apples, pâtés, and cooked, ready-to-eat sliced deli meats.

Species of Listeria can also be found in water, soil, infected animals, human and animal feces, raw and treated sewage, leafy vegetables, effluent from poultry and meat processing facilities, and raw (unpasteurized) milk.

According to some studies, one to 10 per cent of humans may be intestinal carriers of Listeria in their stools.

When a listeriosis outbreak occurs, it can be tough for epidemiologists to identify the food source. This is because the period between exposure to the Listeria bacteria and the appearance of the first symptoms (better known as the incubation period) ranges from about one to 70 days.

Many of us can recall what we ate in the last two or three days, but to go back two or three weeks or as far back as over two months can be a daunting task.

The promise of microbiome research

Listeria monocytogenes can be very hard to eradicate from processing plants. But there may be novel ways for us to tackle this bacterium.

Take the body of research referred to as “microbiome research.” The microbiome of a food, for example, refers to all the microorganisms that are present in that food at a particular point in time.

My lab is trying to isolate different bacteria from the microbiome of unique and/or exotic foods, to see if any of them will have the ability to stop Listeria in its tracks.

In addition, my colleague Dr. Gisèle LaPointe and I will soon purchase a special instrument that will enable us to simulate the human gastrointestinal tract, including the large intestine.

This machine will allow us to study in great detail how pathogenic bacteria such as Listeria monocytogenes interact with the microbiome that is present in human intestines.

Studying the interaction between the bacteria naturally present in the large intestine and Listeria may help us to develop new ways of fighting this dangerous food-borne pathogen.

Since Listeria is so hardy, and it’s so difficult to eliminate it from the environment of processing plants, adding good bacteria to foods to kill off the dangerous ones may be an ideal solution.

How at-risk individuals can avoid Listeria

Foods that are contaminated with the Listeria bacteria will look, smell and taste normal. To avoid contracting listeriosis, here are some tips:

  1. Pay attention to the best-before date on a ready-to-eat food. Remember that once you open, for example, a package of deli meats, the best-before date no longer applies and you should consume the food within two or three days.

  2. Remember to periodically check to make sure that your refrigerator is kept at 4°C or lower. This is because as the temperature increases above four degrees, Listeria is able to grow more quickly.

  3. People who are at-risk for listeriosis should avoid the following foods: Deli meats (unless they are dried and salted or heated until steaming hot), pâté and meat spreads (unless they are frozen, canned or shelf-stable), hot dogs (unless they are heated until steaming hot), raw or unpasteurized dairy products, including soft and semi-soft cheeses such Brie and Camembert and refrigerated smoked salmon.
    Avoidance of pasteurized soft and semi-soft cheeses is also recommended as these can be as contaminated as raw milk cheeses.

Recommendations for the food industry globally

The food industry has made tremendous advances in controlling Listeria monocytogenes in ready-to-eat foods. However, outbreaks still occur.

Special attention needs to be paid to cleaning and sanitising in food processing areas, especially of conveyor belts and equipment.

Continuous sampling of food plant environments for Listeria is also necessary — to give facilities an early warning that there may be a problem.

Retail deli areas have also been pinpointed as a source of the organism. Much of the food contamination with Listeria monocytogenes occurs after a particular food has been heat processed.

The Conversation, authored by Professor of Food Safety, University of Guelph, Ontario, Canada