sourdough bread

Why sourdough bread resists mould

Sourdough bread resists mould, unlike conventionally leavened bread. Now scientists at of the University of Alberta, Edmonton, show why.

During sourdough production, bacteria convert the linoleic acid in bread flour to a compound that has powerful antifungal activity. The research, which could improve the taste of bread, is published in the journal Applied and Environmental Microbiology.

The major benefits from the research are twofold say scientists Michael Gaenzle and colleagues: Firstly, better tasting bread because preservatives can be eliminated from the recipes, and because sourdough bread has a more distinct and richer flavour compared to bread produced with yeast only. 

And secondly, the development of novel tools to control fungi in malting and plant production, via treatment of seeds with the anti-fungal fatty acids.

Genuine sourdough bread differs from ordinary bread in having an extra fermentation step, over and above yeast fermentation. This step is mediated by lactic acid bacteria, typically of the genus Lactobacillus, says Gaenzle.

In the study, “we offered linoleic acid to lactobacilli and screened for organisms producing potent antifungal activity,” says Gaenzle. The investigators then fractionated the metabolites to isolate and identify compounds with antifungal activity.

“The identification was a bottleneck in the research project,” he says. “In collaboration with analytical chemists, we had to develop novel methods for identifying the compounds.”

L. hammesii produced substantial quantities of hydroxylated monounsaturated fatty acids which the researchers found strongly inhibited mould formation. A second antifungal fatty acid produced by cereal enzymes contributes to the antifungal activity of sourdough.

“The two compounds and their formation by cereal or microbial enzymes had been described previously, but their antifungal activity and their generation in food production was unknown,” says Gaenzle.

These new findings, he says, were “a step towards understanding how and why lactobacilli metabolise fatty acids. This could be useful in the long term to improve our understanding of the biology of these organisms.”

Source: American Society for Microbiology

Journal Reference:

  1. B. A. Black, E. Zannini, J. M. Curtis, M. G. Ganzle. Antifungal hydroxy-fatty acids produced during sourdough fermentation: microbial and enzymatic pathways, and antifungal activity in bread. Applied and Environmental Microbiology, 2013; DOI: 10.1128/AEM.03784-12